Introduction：
Hybridoma technology is a technology featuring effective usage of innate functions of both immune cells and cancers to generate monoclonal antibodies (mAbs)-producing hybridoma cell-lines against specific antigens of interest. Such technology has opened remarkably new approaches to the therapeutic mAb discovery, diseases diagnosis, as well as prevention. With our talent scientist team and excellent technical supports from both local and international antibody specialists, we have developed our own antibody drug discovery platform, and provide a full range of services in custom monoclonal antibody production including immunization, hybridoma development, antibody discovery, antibody functional screening assays, antibody engineering and antibody expression. With our optimized immunization (include protein, whole cell, DNA immunization); high throughput screening for affinity measurements, epitope binning and isotyping; and a series of reliable functional screening assay, we promise to deliver up to 10 high quality functional hybridoma clones, assuring our customers have a good start for their research in antibody drug discovery. In order to serve our customer better, we offer high quality customized “one stop solution” from antigen production to hybridoma generation and characterization, in which the protocol is adjustable based on the actual requirements of clients or the progress of the project.
Competitive Advantages：
• Proprietary immunization technology: UniversalAntigenTM
• One Stop solution: Complete strategies from immunization to hybridoma generation and characterization.
• High throughput screening
• Comprehensive functional assay: Professional in antibody affinity ranking, affinity measurement, cross-reactivity and other in-vitro biological analysis
Product/ Service details：
|
Product/ Service |
Deliverable |
|
Immunization |
√ Immunization of mice, transgenic mice or rat (accoding to customer’s request) √ Serum test √ Maintain immunized (6 months) |
|
Cells fusion, Screening |
√ Cells fusion √ Primary screening √ Clones amplification/ Supernatant collection √ Secondary screening √ Positive clones selection and freezing |
|
Cells screening and isotyping |
√ All cells isotyping based on epitope-binding groups √ Bioactivity evaluation of all positive clones (50-100 clones) |
|
Antibody production (Small Scale) |
√ Small scale antibody production (50-100 clones/ Supernatants/ 1-5mg purified antibody/ clone) |
|
First Delivery |
√ Supernatants/ purified mAb (For in vitro functional assay and affinity analysis) |
|
Functional Assay (Optional) |
√ Functional assay provided according to customer’s request |
|
Subcloning, Amplification, Cryopreservative |
√ Subcloning and screening √ Clone freezing (several vials for each clone) |
|
Final delivery |
√ 2 subclones for each parental clone √ 2 cell-lines for each subclones √ 5mL supernatants √ Full report |
Consulting Information：
Please contact us by email or telephone for the specific service price and content.
Email: xilinwu@abrevbio.com & bd@abrevbio.com
Phone: 18805158739
